摘要
应用体外纯培养的贾第虫滋养体免疫ＢＡＬＢ／ｃ小鼠和常规杂交瘤细胞技术，制备特异的抗贾第虫单克隆抗体（１Ｅ２，１Ｇ５），以其与贾第虫滋养体相互作用，均发现具有明显的抗贾第虫效果，生长抑制率分别为３５％和６３．４％；将此单抗加入正常培养液中，其含量与抑制率呈现明显的剂量反应关系，相关系数分别为０．９７６４和０．８９８７；单抗经滋养体吸收后，对贾第虫的生长抑制作用消失；经补体灭活前后的豚鼠血清对单抗的抑制作用无影响。
Monoclonal antibodies(McAbs)were generated by fusion of SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with Giardia lamblia trophozoites.To define the effect of the McAb on Giardia lamblia ,trophozoites were subcultured axenically after contact with McAb for 1 h at 37℃.The McAbs 1G 5 and 1E 2(both IgG 1)were able to inhibit the growth of trophozoite at the rate of 63.4% and 35%, respectively.The inhibitory effect was decreased along with the decreasing quantity of McAb(from dilution 1∶45 to 1∶225)added to the TYI S 33 medium.The actions of McAbs could be eliminated by absorption with trophzoites and not be influenced by complement in fresh guinea pig serum,thus the actions of McAbs appeared to be specific and complement independent.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1994年第1期56-58,共3页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
贾第虫
单克隆抗体
Giardia lamblia, monoclonal antibody
