摘要
应用反转录－聚合酶链式反应（reversetranscription－polymerasechainreaction，RT-PCR）方法，测定出一氧化氮合成酶（nitricoxidesynthetase，NOS）mRNA不仅在心血管系统细胞中广泛存在，并且发现内皮素可以促进血管平滑肌细胞中NOSmRNA的表达（＋34．8％），增高血管平滑肌细胞中NOS酶活性（＋74％）。结果提示，由内皮素引起的血管平滑肌细胞中NOS基因表达和NOS活性的增加，可能是高血压中的一个重要的负反馈因素。
With reverse transcripti0n-polymerase chain reaction (RT-PCR),it has been found that NOS mRN;\ is widely distributed in cells of car<liovascular system such as t'ardiomyocytes, endothelia1 ce]ls, vascular smooth musclc cel1s (VSMC ). When ET (1O-' mol/L ) was added to cu1tured VSMC, it significant1y increased the NOS mRNA leve1 in VSMC (+ 34. 8% ). By measuring the enzyme activity of NOS in cultured VSMC,it was f0und that when VSMC was treated with ET (2 X 10wv mol/L) l NOS activati0n was significantly increased (79% ) compared to the contr0l. These data show that the increased expression of NOS gene inVSMC induced by ET may be an important regulat0ry factor for hypertension.
关键词
内皮素
一氧化氮
合成酶
高血压
endothelin
nitric oxide synthetase
vascular smooth muscle cell
hypertension
