摘要
目的:通过自身对照的方法研究实验狗种植体周围炎IL- 8水平和临床指标的变化。方法:通过丝线栓结法建立狗的种植体周围炎的动物模型,采用双抗体夹心酶联免疫吸附法(ABC ELISA法)测定5 条狗的种植体周围炎28 颗、自身对照的健康种植体27颗的龈沟液中IL- 8含量、IL- 8总量。同时测量临床病理指标(菌斑指数PI,探诊龈袋深度PD,牙龈指数GI,松动度MOB)。运用SPSS12.0统计软件进行Mann Whitney U显著性检验。结果:在种植体周围炎模型组和自身对照的健康种植体模型组之间IL- 8含量、IL- 8总量、GCF量均存在着统计学差异(P<0.01),并且发现GCF、IL- 8含量与临床病理指标之间有着显著正相关(P<0.01)。结论:IL- 8参与种植体周围组织炎症的免疫调节,并且有可能作为评价种植体组织健康程度的指标。
Objective: The changes of IL-8 levels and clinical indexs were compared between experimental peri-implantitis and healthy case of dogs. Methods: The animal model of peri-implantitis of dogs were established through silk knot. The contents and total amounts of IL-8 cytokine within gingival crevicular fluids(GCF) between 28 experimental peri-implantitis implants and 27 own controlled healthy implants on 5 dog models were examined by double-antibody sandwich ABC-ELISA method. At the same time,the clinical indexes about gingiva ,such as PI(plaque index)、PD(probing deepth)、GI(gingiva index) and MOB(mobility) were surveyed.The data were analyzed by software package SPSS 12.0. Results: Significant differences of the total amounts of IL-8、contents of IL-8 and GCF volume between the two groups were displayed (P< 0.01), GCF volume and contents of IL-8 were positively correlated with the clinical indexes(P<0.01).Conclusion:IL-8 was concerned with the immunological response of peri-implantitis ,and it could be used to evaluate the healthy condition of the peri-implantitis.
出处
《中国临床医学》
北大核心
2005年第2期321-323,共3页
Chinese Journal of Clinical Medicine
基金
上海市科学技术委员会资金赞助(编号024119072)
