摘要
本文用推挽灌流技术和特异性放射免疫分析法，测定大鼠视前区灌流液中GnRH和γβ-内啡肽的含量，以观察腹腔注射纳洛酮和电针对视前区GnRH和γβ-内啡肽释放的影响，结果表明，卵巢切除组大鼠GnRH的基础含量明显高于正常组大鼠（P＜0.02），腹腔注射纳洛酮30分钟后，卵巢切除组大鼠视前区GnRH含量比注射前明显升高（P＜0．01），而γβ-内啡肽含量未见明显改变，注射纳洛酮对正常组大鼠GnRH含量无明显影响。电针对卵巢切除组和正常组大鼠视前区GnRH及γβ-内啡肽含量均无明显影响，以上结果提示正常生理情况下，雌激素的反馈抑制可能是视前区GnRH释放的主要调节机制，巢卵切除后，雌激素的反馈机制被去除，中枢内阿片肽对视前区GnRH的释放抑制转为其主要的调节机制。
By means of push-pull perfusion technique and the special radioimmunoassay we determined the GnRH concentration of the perfusates from preoptic area (POA) in ovariectomized (OVX) and intact (INT) female rats, to observe the influence of naloxone and electroacupuncture (EA) on the release of GnRH and β-endorphin from POA. The results show that the basic GnRH level of POA perfusate in OVX was 271. 8±77. 5pg/ml and that in INT was 52.4±11. 1pg/ml. There was a significant difference between the basic levelsof two groups (P<0.02,n = 12 ). No difference of β-endorphin basic level in OVX and INT was found. The GnRH level of POA perfusate in OVX was increased by 146.6±46. 5pg/ml (P<0.01, n = 12 ) 30 minutes after intraperitoneal administration of naloxone, but no obvious change was found in INT following naloxone administration. EA treatment had no effect on the release of GnRH and β-endorphin in both of OVX and INT. The results suggest that the negativefeedback of estrogen may be a major regulative factor of GnRH under the normal condition and after the abolition of estrogen feedback induced by ovariectomy the centrol opioid peptide might be the main regulative mechnism for GnRH.
出处
《针刺研究》
CAS
CSCD
1994年第2期31-34,共4页
Acupuncture Research
基金
国家自然科学基金
