摘要
利用海藻酸钠固定化酿酒酵母细胞和流化床生物反应器进行介质循环发酵。反应器中固定化胶体最适体积分数(φ)为0.40(v/v)。在给定固定化胶体体积分数(φ=0.40),给定发酵温度(10℃)和循环比(n=5)的条件下,研究了循环流速对主发酵周期和对嫩啤酒双乙酰水平的影响。结果表明,发酵周期随流速的增加而减少,而双乙酰浓度则随流速的增加而提高。当停留时间τ=2.8h。发酵周期T(=nτ_t)为14h,嫩啤酒中双乙酰浓度为0.5ppm。
A fluidized-bed bioreactor containing yeast cells immobilized on calcium alginate gel was operated by using a circulating wort system. The optimum volume fraction of gel beads was found to be 0.40(φ, v/v) for fermentation. Under the following conditions: the volume fraction of gel beads at φ=0.40, the fermentation temperature at 10℃ and the ratio of circulation at n=5, the effects of the flow rates of wort oft the period of primary fermentation and diacetyl levels in green beer were studied. Experimental reslts has indicated that the period of primary fermentation decreased with increasing the flow rate of wort, whereas the concentration of diacetyl increased with increasing the flow rate of wortoThe period of primary fermentation and the concentration of diacetyl were found to be 14 h and 0.5 ppm respectively, when the residence time in the reactor (τ_i) was 2.8h.
出处
《生物工程学报》
CAS
CSCD
北大核心
1989年第4期322-327,共6页
Chinese Journal of Biotechnology
关键词
酿酒酵母
固定化
流行床
双乙酰
Brewer's yeast
fluidized bed
immobilization
diacetyl
