摘要
目的观察缺血预处理对大鼠肝缺血再灌注后即早基因c-fos、c-jun表达的影响。方法采用大鼠原位部分缺血再灌注模型,96只SD大鼠随机分为缺血再灌注组(IR),缺血预处理组(IPC),每组又分为8个亚组(n=6),于复灌后0、0.5、1、2、4、8、12和24h取材,应用RT-PCR法检测各组c-fos、c-junmRNA的表达,流式细胞仪检测Ki67和Sub-G1。结果与IR组相比,IPC组血清ALT、AST在复灌后的0.5￣8h组明显降低(P<0.05);Ki67在复灌后的0.5、1和2h明显升高,24h明显降低(P<0.05);Ap指数在复灌后的1h以上明显降低(P<0.05);IPC组c-fos和c-junmRNA的表达较IR组低,其中c-jun在0.5、1和2h组明显降低(P<0.05)。结论缺血预处理能有效地保护肝脏免受缺血再灌注造成的损伤,这种保护效应的机制可能与影响即早基因的转录有关。
To investigate the effects of preconditioning on expression of immediate early genes c-fos and c-jun following hepatic ischemia/reperfusion (IR) and its roles in cellular regeneration and apoptosis. Ninety-six Wistar rats were randomly divided into IR group and IPC group, each group was divided into eight sub-groups (n =6). The model of partial liver ischemia/reperfusion was used. Rats subjected to 60 min liver ischemia, preceded by 10 min preconditioning. After 0, 0.5, 1, 2, 4, 8, 12 and 24 h reperfusion, the serum and liver tissue in each group were collected to detect the serum ALT/AST , liver histopathology, expression of c-fos and c-jun mRNA. Flow cytometer was used to detect Ki67 and Sub-G1 as the quantity indicators of cell regeneration and apotosis respectively. Compared with group IR, group IPC showed significantly lower ALT/AST level in sub-group 0.5 h to sub-group 8 h (P <0.05); Ki67 elevated significantly at 0.5, 1, 2 h, but decreased significantly at 24 h (P <0.05); Ap index decreased significantly after 1h reperfusion(P <0.05); Expression of c-fos and c-jun mRNA were low, especially c-jun at 0.5, 1 and 2 h after reperfusion. [Conclusion] Ischemic preconditioning can protect liver cells against ischemia/reperfusion injury, this protective effect may be related to influence transcription levels of c-fos and c-jun.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第6期843-846,共4页
China Journal of Modern Medicine
