摘要
建立了快速部分提纯和测定热带假丝酵母中肉毒碱乙酰转移酶 (carnitine acetyltransferase, CAT) 酶活的方法. 并考察了重组热带假丝酵母的CAT酶活、产酸能力及烷烃转化率. 结果表明, 重组菌的 CAT酶活比原始菌降低了45%, 胞内二元酸通过β 氧化的无益消耗得到有效抑制. 相应地, 重组菌的产酸能力和烷烃转化率分别提高了11%和12%.
A rapid method of partially purifying carnitine acetyltransferase (CAT) from Candida tropicalis and determination of its enzyme activity was established. The characteristics of CAT-disruption recombinant of Candida tropicalis were also studied. It was confirmed that the specific activity of CAT of this recombinant was approximately half of that of its corresponding wild type. Therefore, the β-oxidation pathway of dicarboxylic acid was effectively suppressed. Correspondingly, the dicarboxylic acid productivity of this recombinant was increased by 11% and its alkane conversion rate was also increased by 12% in average.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2005年第3期511-517,共7页
CIESC Journal
基金
国家重点基础研究发展规划项目(2003CB716007)
国家自然科学基金重点项目 (20036010).~~
