摘要
目的:建立测定补肾壮阳药中枸橼酸西地那非含量的方法。方法:采用反相高效液相色谱法,DiamonsilC18柱(150mm×4.6mm,5μm),0.05mol/L磷酸(三乙胺调pH至3.0±0.1)-甲醇-乙腈(55∶25∶20)为流动相,流速1.0mL/min。结果:其工作曲线的线性范围为0.04～1.00μg/mL,相关系数r=0.9995,平均回收率为96.3%(n =6)。结论:所建立的方法准确、可靠,可应用于检测补肾壮阳药中枸橼酸西地那非的含量。
Objective:To set up a practical method for determination of sildenafil citrate in invigorant.Methods:Reversed phase high performance liquid chromatography was used.The operating condition were Diamonsil C18 column,Phosphoric acid and triethylamine-Methanol-acetorutrile=(55 ∶25 ∶20) as mobile phase with flow rate 1.00 mL/min and UV detection at 290 nm.Results:The linear range of the calibration curve of sildenafil citrate were from 0.04～1.00 μg with a correlation coefficient of 0.999 5.The average recovery was 96.3%.Conclusion:The method was accurate,realiable and can be used for determination of sildenafil citrate in invigorant.
出处
《中国药业》
CAS
2005年第3期40-41,共2页
China Pharmaceuticals
