摘要
该项研究确立了聚乙二醇（ＰＥＧ）沉淀比浊法，是检测水貂血清中循环免疫复合物（ＣＩＣ）的理想方法。该方法的最适条件是：ＰＥＧ浓度为５％；缓冲液浓度为０．１Ｍ，ｐＨ８．４；作用温度为４℃，作用时间为１ｈ；室温２０℃放置时间为３０分钟。用该方法检测临床上健康、经阿留申病（ＡＤ）对流免疫电泳（ＣＩＥＰ）确认为阴性的水貂血清２００份、阿留申病阳性水貂血清６００份，其结果（ＯＤ值，Ｘ±ＳＤ）为：健康水貂０．０３４±０．０２３，阿留申病水貂０．２４１±０．１５２。经统计学处理，健康水貂与阿留申病貂的血清ＯＤ值之间，差异极显著（Ｐ＜０．０１），说明水貂ＡＤ的发病与ＣＩＣ密切相关。ＰＥＧ沉淀比浊法具有快速、简便、不需要特殊设备和重复性好等特点。
In this study, it was developed that polyethylene glycol(PEG) precipitation turbidimetry was th reasonable method for detecing the circuitingimmune complexes(CIC) in mink sera. This method was carried out throughkeeping the mixture of serum and 5% PEG(prepared with PH 8.4, 0.1Mborate buffer solution)for 1h at 4℃ and 0.5h at 20℃.The sera sample of 200 clinical healthy AD. CIEP negatiye minks and600 positiye minks with Aleutian disease was detected with t his method.The OD valuo (X±SD) of healthy minks was 0.034±0.023 and that of ADminks was 0.241±0.152.There was st gulf leant diffierence (p<0.01) betweenthe OD value of healthy minks and that of AD minks by statistical comparison.The result suggest that the CIC are closely Correlat ed to pat hogenesis of AD.PEG precipitat ion turbidimetry are rapid,simple, not needing specialequipment and good repeat ion. The development of this assay method issignificant to the research for pathogenesis mechanism and immune of ADin mink.
出处
《特产研究》
1994年第2期1-4,共4页
Special Wild Economic Animal and Plant Research
基金
"八五"农业部畜牧业重点科研专题
国家自然科学基金
