摘要
本研究探索采用电击法导入荧光标记物的条件。发现钙黄素荧光标记物可以导入平菇原生质体,脉冲强度在0～1500v/cm条件下,导入率呈线性增长,成活率则下降,两种观测指标呈相反趋势。平菇原生质体的最适电击脉冲强度为1500v/cm(3×1.1cm)。
This study explores the condition of introducing fluorescent mark material by electrostimulation. The result indicats that calcein fluorescent mark material can be introduced into protoplasts of Pleurotus ostreatus. The introduing rate increases linearly while the survival rate decreases when pulse height are under the condition of 0-1500v/cm. The best electrostimulation pulse height was 1500v/cm (3@1.1cm).
出处
《食用菌学报》
1994年第1期50-52,共3页
Acta Edulis Fungi
关键词
平菇
原生质体
电击导入
钙黄素
Pleurotus ostreatus
Protoplasts
Electrostimulation introduing
Calcein
