摘要
获得了马尾松毛虫核型多角体病毒（ＤｐＮＰＶ）包涵体在Ａｇ胶中的ＳＥＲＳ谱。包涵体是通过膜蛋白的ＣＯＯ－和ＮＨ２基团吸附在Ａｇ表面上，Ｔｒｐ和Ｐｈｅ残基侧链接近Ａｇ表面，Ｔｙｒ残基侧链远离Ａｇ表面，ＳＥＲＳ谱中没出现ＡｍｉｄｅⅠ和ＡｍｉｄｅⅢ带。强的低波数谱带（２３８ｃｍ－１）的出现表明包涵体与Ａｇ表面发生了明显的化学吸附。
The surface-enhanced Raman scattering (SERS) spectra of the inclusion body of DpNPV were studied. Results showed the inclusion body of DpNPV was adsorbed on Ag colloidal particles through COO- and NH2 groups of inclusion body envolope protein. The side-chains of Trp and Phe residues were closed to the surface of silver. The side-chain of Tyr residus was away from the surface of silver. No Amide Ⅰand Amide Ⅲ bands were observed in the SERS spectra. The appearance of a strong low-frequency band(238cm-1) indicates appreciable chemisorption of the inclusion body on the surface of silver.
出处
《生物物理学报》
CAS
CSCD
北大核心
1994年第4期525-528,共4页
Acta Biophysica Sinica
基金
国家自然科学基金
