摘要
生物功能分子的二维有序化组装是分子工程学的重要内容。本文通过构建有生物素受体的人工模型膜，对亲和素与生物素这一具有极强亲和力的系统之间的特异性相互作用的某些制约因素进行了探讨．应用ＬＢ膜技术结合激先椭圆偏振术和表面等离激元谱技术，较深入地研究了蛋白质与脂单层膜发生的非特异性吸附，特异性结合以及结合的侧向空间位阻效应．实验结果表明，膜表面电荷对非特异性吸附的速率有很大的影响，而对最终的蛋白吸附量影响不大；非特异性的吸附可以通过二阶阳离子的脱附而去除；受体蛋白质与配体间的特异性结合受到侧向空间位阻效应的制约．
Two-dimensional assmble of biological molecules is one important field in field in the molecular engineering. We.studied the interaction of avidin with phospholipid model membranes con taining biotin-conjugated phospholipids. Using LB technique together with sarface sensitive techniques (such as eilipsometry and sarface plasmon spectroscopy), we studied the nonspecific adsorption and specific binding of avidin to phospholipid lamella, and the effect of the lateral steric hindrance.The primary conclusions of the present work are as following first, the charges of membrane sarface effect the rate of protein adsorption, but do not effect the amount of protein ultimately adsorbed second, the nonspecific adsorption of avidin can be detached by adding a suitable amount of bivalent alkaline-earth metal ions; third, the binding of avidin to membrane bound model receptor is astricted remarkable by the latelal steric hindrence.
出处
《生物物理学报》
CAS
CSCD
北大核心
1994年第2期203-209,共7页
Acta Biophysica Sinica
基金
国家自然科学基金
关键词
人工膜
亲和素
蛋白
生物膜
Molecular assemble Artificial membrane Ellipsometry SPS
