摘要
观察了肽类生长因子对２ＢＳ细胞中抗癌基因表达的影响。结果表明，ＥＧＦ或ＦＧＦ均可明显诱导２ＢＳ细胞中Ｒｂ基因的表达，其幅度分别为３０５％、２４３％；ＥＧＦ也可诱导２ＢＳ细胞中ＴＧＦβ１基因表达增加３０－５０％，但ＦＧＦ对ＴＧＦβ１的表达无明显影响；ＥＧＦ或ＦＧＦ对ｐ５３基因的表达均无明显诱导作用。上述结果为生长因子作用机制研究及生长因子与抗癌基因的关系提供了新的线索。
Expression of Rb, p53 and TGFβ1 gene in 2BS cells after EGF or FGF stimulation was analyzed by total RNA dot blot and Northern blot. The results indicated that the expression of Rb gene was increased 2. 5-3 fold by both EGF and FGF, TGFβ1 mRNA was induced only by EGF,and p53 mRNA was induced by neither EGF nor FGF. Cellular signalling pathways may participate in the induction process. This research may provide a new clue to understand the molecular mechanism of growth factors.
基金
国家自然科学基金
卫生部科研基金
关键词
表皮生长因子
成纤维
肽
细胞生长因子
抗癌基因
Epidermal growth factor
Fibroblast growth factor
Anti-oncogene
TGFβ1 gene
