摘要
采用体内裸ＤＮＡ基因治疗帕金森病（ＰＤ）取得显著疗效，将酪氨酸羟比酶（ＴＨ）基冈表达质粒与Ｌｉｐｏｆｅｃｔｉｎ形成的复合物立体定位注射于ＰＤ模型鼠纹状体．显著改善了ＰＤ鼠的不对付旋转行为．
In vivo naked DNA gene transfer method wasused in gene therapy of Parkinson's disease(PD). The complex of rat tyrosine hydroxy-lase (TH) expression plasmid and Lipofectinwas injected stereotaxically into striatum of PDrat model. The asymmetric rotational behaviorwas reduced substantially and quickly. On thethird day after injection, drug-induced rotationdecreased 50% compared with pretreatmentscores. Immunohistochemical staining showedTH-positive nerve cells in striatum of injectionside, which indicated that TH gene was up-taked and expressed by nerve cells. These pre-liminary results have general implications forthe application of naked DNA transfer tech-nique in gene therapy of human neurologicaldisease and specific implications for PD.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1994年第4期369-369,289,共2页
Progress In Biochemistry and Biophysics
关键词
基因治疗
酷氨酸羟化酶
震颤性麻痹
Parkinson's disease, gene thera-py, tyrosine hydroxylase
