摘要
本文观察了缺血性肾损伤中肾小管上皮细胞死亡的另一种形式—Ａｐｏｐｔｏｓｉｓ。通过钳夹肾蒂造成小鼠缺血冉灌注肾损伤模型，结果发现，缺血５ｍｉｎ再灌注１２～２４ｈ肾小管上皮细胞并不出现坏死，但可发生Ａｐｏｐｔｏｓｉｓ现象。随着缺血时间的延长，缺血３０ｍｉｎ再灌注６～２４ｈ，或缺血４５ｍｉｎ再灌注６～１８ｈ，肾小管和间质出现Ａｐｏｐｔｃｓｉｓ小体，肾脏ＤＮＡ电泳像呈“梯形结构”。梯形结构的出现持续２４ｈ后消失。结果提示，缺血再灌注肾损伤中存在Ａｐｏｐｔｏｓｉｓ机现的参与，Ａｐｏｐｔｏｓｉｓ小体和梯形结构可作为缺血性肾损伤的早期观察指标。
A multiparanietric analysis to demonstratethat even breif poriod of
ischemia can initiateextensive lase in a rat kidney through the processof Apoptosis during
early reperfusion.Microscopic exaniination of mouse renal tissuessubJecteil to a 5-, 30-, or 45-
minute periodcomplete ischeinia showed the presence ofapoptotic bodies .both within and
occasionallybetween renal tubular, aplwaring as early as 6houro after reperfusion and
increasulg innumbers at 12 hours. Furtherniore, DNAextracted forrn such reperfusion renal
tissuedemonstrated the appearance of a distinct“ladder pattern” of DNA fragments
afterelectrophoresis in agnase gels. It was sugsestedthat renal reperfusion injury after
ischemia couldinitiate a form of cell death-Apoptosis that isdrastically different from eellular
necrosisinduced by prolonged severe ischemia.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
1994年第4期262-264,共3页
Chinese Journal of Nephrology,Dialysis & Transplantation
关键词
肾损伤
缺血性
程序化
细胞死亡
peptosis
necrosis
ischemia
ladder parttern
reperfusion
renal injury
