摘要
探讨了用高效毛细管电泳法（ＨＰＣＥ）测定肾上腺素（Ｅ）、去甲肾上腺素（ＮＥ）的最佳分离条件，即以ｐＨ６．０的硼酸溶液加入０．１ｍｏｌ／ＬＮａＣｌ和２０％丙二醇为载体缓冲液，ＮＥ与Ｅ的浓度在０．０１～０．０５μｇ／ｍｌ内与峰面积之间有良好的线性关系（ｒＮＥ＝０．９９９０，ｒＥ＝０．９９８７），重现性结果为日内ＲＳＤＮＥ＝３．５２％，ＲＳＤＥ＝４．８１％；日间ＲＳＤＮＥ＝６．４２％；ＲＳＤＥ＝７．２７％。
This paper described the separation and determination of norepinephrine(NE)and epinephrine(E) by high performance capillary electrophoresis(HPCE).The effects of buffer PH, salt and ethylene glycol on the peak shape, retention time and the separation results were studied.The promising running buffer for the analysis of NE and E was pH6.0 boric acid containing 0.1mol/L NaCl and 20% ethylene glycol. The consentration of NE and Ewas linear to the peak area in the range of 0.01～0.05μg/ml (rNE= 0.9990,rE=0.9987). The RSD of the reproducibility of NE and E was 3.52%, 4.81%(in the same day) , 6.42%, 7.27% (in different days).
出处
《山东医科大学学报》
1994年第4期347-349,共3页
Acta Academiae Medicinae Shandong
关键词
肾上腺素
去甲肾上腺素
测定
高效毛细管电泳
Epinephrine
Norepinephrine
High performance capillary electrophoresis
