摘要
用化学偶联的双特异性抗体复合物－αＴＴ－αＩｇＭ通过体外实验。观察其抗原递呈能力。结果显示：αＴＴ－αＩｇＭ存在时，可使非特异性Ｂ细胞的抗原递呈效应显著提高，所需抗原浓度较不加αＴＴ－αＩｇＭ的对照组低１０００倍左右，与抗原特异性Ｂ细胞的抗原递呈效应相似。抑制试验显示：羊抗鼠ＩｇＭ，鼠ＩｇＭ能抑制αＴＴ－αＩｇＭ对非特异性Ｂ细胞抗原递呈作用的增强效应，表明Ｂ细胞膜ＩｇＭ在抗原递呈中起关键性作用。本研究结果表明：αＴＴ－αＩｇＭ的存在确能模拟抗原特异性Ｂ细胞的高效抗原递呈功能。
By two independent assay(T cell proliferation &IL-2 secretion)the effect of
bispecific antibody complexby chemical conjugated-aTT-aIgM on presentation of antigen was
investigated,In the presence of aTT-aIgM.the abilityto present antigen of non-specific B cell was
significantly enhanced and the antigen(TT) was presented at concentration10￣3-fold lower than
that by non-specific B cell without aTT-aIgM or by M.but just the same as the antigen-specific
Bcell.We also observed that the enhanced presentation was significantly inhibited by mouse
IgM or anti-mouse-IgM. Theseresults indicated that high efficiency presentation of
antigen-specific B cell can be mimicked by aTT-aIgM.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1994年第3期154-157,161,共5页
Immunological Journal
基金
国家自然科学基金
卫生部科学基金
纽约中华医学基金(CMB)
关键词
双特异性
抗体
B细胞
抗原
递呈
Bispecific antibody complex,B
lymphocyte, Antigen presentation
