摘要
本文报道了肝癌单克隆抗体Ａ２４－表阿霉素结合物的制备及其对肝癌细胞ＳＭＭＣ－７７２１的体外杀伤作用。先用高碘酸钠把葡聚糖氧化成多醛基葡聚糖，再以此为中介体连接单抗与表阿霉素。结合物中抗体与药物摩尔比为１：４０。单抗活性保存良好。该结合物对肝癌细胞的毒性显著高于游离药物及无关抗体结合物，而对非靶细胞的毒性则明显低于游离药物。说明结合物对肝癌细胞有较强的选择性杀伤作用，有可能用于肝癌导向治疗。
A conjugate of epirubicin with murine McAb A24 against human hepatoma was prepared and its cytotox-icty to hepatoma cell line SMMC-7721 was studied. Dextran T-40 was oxidized to polyaldehyde-dextran (PAD)withsodium periodate. Eprrubicin was then linked covalently with A24 using PAD as a multivalent carrier. The conjugate (A24:epirubicin molar rate,1:40) retained full antibody activity. The cytotoxicity of the conjugate against hepatomacell line SMMC-7721 was significantly higher than that of free epirubicin or normal murine immunoglobulin conjugate. The cytotoxicity of the conjugate was significantly lower than that of free epirubicin against non-target cell Hep- 2.Ourresult suggested that the conjugate exhibited highly selective cytotoxicity to hepatoma cells.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1994年第1期40-43,共4页
Immunological Journal
