摘要
以聚乙二醇(PEG,MW6000)沉淀法部分纯化的猪瘟兔化弱毒中国株(SFV-C)免疫BALB/C小鼠,取其脾脏制备脾细胞与SP2/0骨髓瘤细胞融合,经ELISA检测和有限稀释法克隆化筛选出9株对SFV特异的单克隆抗体(McAb)杂交瘤细胞。它们产生的McAb仅对SFV-C株发生特异性反应,而与SFV-S及BV DV Oregon株不发生反应.相加试验表明,9个McAb分别针对不同的抗原决定簇。所有的McAb均不具有沉淀反应特性。
Nine hybridoma cell lines secreting monoclonal antibody(McAb) against lapinized Chinese strain of swine fever virus (SFV-C)were established by means of the fusion between mouse myeloma SP2/0cell and spleen cell from BALB/C mouse which were immunized withSFV-C antigen partly purified by PEG precipitation. The McAbsreacted only with SFV-C strain, but did not with SFV Shimen strainand bovine viral diarrhoea virus Oregon strain. All the McAbs arespecific for different antigen determinants, respectively, and haveno precipitation character.
关键词
猪
猪瘟病毒
单克隆抗体
兔化弱毒
swine fever virus
lapinized chinese strain
monoclonal antibody
