摘要
建立了由蛋白质、磷酸氢二钾、磷酸二氢钾、聚乙二醇所组成的双水相体系的平衡浓度分析测定方法,实验测定了6种蛋白质分子在25℃下的液液平衡分配数据,共23组.将Baskir等的吸附晶格模型和Pitzer的长程静电项相结合,扩展用于含电解质-高聚物双水相体系的热力学计算,仅用7组实验数据进行关联,得到2个吸附能参数,预测了其它16组蛋白质分配系数,结果满意。并提出了蛋白质分配系数与双水相成相物质在两相间浓度差的经验关系式。
A quantitative analytical method for the study of the LLE in protein - K2HPO4-KH2PO4- PEG aqueous two - phase systems has been established. The partition coefficients of six proteins in K2HPO4- KH2PO4- PEG4000 aqueous two - phase systems were measured experimentally at 25 ℃. The Baskir adsorption lattice model combined with the Pitzer long range electrostatic term was proposed to calculate the partition coefficient of the proteins for the aqueous two-phase systems including electrolytes. The 16 sets of distribution data of proteins can be predicted successfully by use of two adsorption parameters regressed from 6 sets of experimental data. An empirical formula correlating partition coefficient and tie line length was proposed.
出处
《化工学报》
EI
CAS
CSCD
北大核心
1994年第5期515-522,共8页
CIESC Journal
基金
国家自然科学基金资助项目
关键词
磷酸钾
聚乙二醇
蛋白质
双水相
potassium hydrogen phosphate, potassium dihydrogen phosphate, polyethylene glycol, protein, partition coefficient, aqueous two - phase, adsorption lattice, model
