摘要
应用猪痢疾密螺旋体（Ｔ．Ｈ．）免疫血清结合葡萄球菌甲蛋白（ＳＰＡ）与Ｈ１０、２－５、Ｘ_（２３）、Ｃ_（１７）等Ｔ．Ｈ．菌株进行协同凝集试验，均出现明显凝集反应。而免疫血清结合不含ＳＰＡ的菌株、无特定病原（ＳＰＦ）血清结合含ＳＰＡ的菌株均无凝集反应。免疫血清结合ＳＰＡ与７种肠道菌均无凝集反应。免疫血清用量以０．２ｍｌ为合适。最低用菌量为３０倍稀释，即每个视野Ｔ．Ｈ．约４０个菌体。
By using staphylococcal proteni A binded with Treponema hyodysenteriae immune serum for identification of the local T.H.strains i.e.,H10,2-5,X23,C17,Z81;all were found with positive agglutination.Whereas wood 46 binded T.H.immune serum and cowanⅠstrain binded SPF pig serum,no agglutination was seen. The minimum bacterial reagent which was T.H.tryptose broth culture could be diluted 30 time with about 40 organisms per microscopic oil immersion field.There was no common antigenicity observed between T.H.and seven species of enterobacteriacea.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1994年第3期274-277,共4页
Journal of Huazhong Agricultural University
关键词
协同凝集试验
猪病
痢疾
蜜螺旋体
treponema hyodysenteriae(T.H.),staphylococcal protein A,coagglutination
