摘要
作者在大鼠脑干脑片旁巨细胞外侧核（ＰＧＣＬ）区，用多管微电极技术观察了微电泳谷氨酸（Ｌ－Ｇｌｕ）及其拮抗剂ＤＬ－２－氨基－５－磷酸戊酸（ＡＰ５），对神经元自发放电的效应及ＡＰ５对Ｌ－Ｃｌｕ作用的影响。在５１片脑片ＰＧＣＬ区共记录１３６个自发放电稳定的神经元，主要呈重复放电样式。Ｌ－Ｇｌｕ和ＡＰ５对自发放电均有兴奋、抑制和无影响三种效应，各占所测试神经元数的８０．６５％、８．０６％、１１．２９％（Ｌ－Ｇｌｕ）和２８．００％、８．００％、６４．００％（ＡＰ５）。兴奋反应呈量效依赖关系。ＡＰ５部分地阻断部分神经元（７１．８７％）对Ｌ－Ｇｌｕ的兴奋作用。结果表明大鼠离体脑片ＰＧＣＬ区神经元自发放电样式主要是重复放电，并从细胞水平上提示ＰＧＣＬ区有起递质作用的内源性兴奋性氨基酸（ＥＡＡ），其神经元上有Ｎ－甲基－Ｄ－天冬氨酸（ＮＭＤＡ）和非ＮＭＤＡＥＡＡ受体亚型。
We studied the
effects of iontophoreticapplication of L-glutamic acid(L-Glu)and its antago-nist
DL-2-amino-5-phosphonovaleric acid(AP5)onthe spontaneous discharge of the nucleus
paragiganto-cellularis lateralis(PGCL)neurons and the influenceof AP5 on L-Glu effect in
brainstem slice in rat withthe multibarrel microelectrode techniques. One hun-dred and thirty six
units with stable spontaneous dis-charge were recorded in 51 rat brain slices, their dis-charge
pattern being primarily a repetitive one. BothL-Glu and AP5 showed three kinds of effect on
thePGCL neuronal spontaneous discharge:excitatory , in-hibitory and no-effect,with the
percentage of80.65%,8.06%,11.29%for L-Glu and 28. 00%,8. 00%, 64. 00% for AP5 of the neurons
tested,re-spectively. The excitatory effect showed a dose-re-sponse relationship. AP5 could
block partially the exci-tatory effect of L-Glu on most neurons tested(71.87%).The results
indicated that the neurons in PGCLin rat brain slice in vitro principally showed a sponta-neous
dicharge with a repetitive firing pattern,andsuggested that at cellular level there existed
endoge-neous excitatory amino acid as neurotransmitter in PG-CL and the neurons there
possessed EAA receptors ofbotb N-methyl-D-aspartate(NMDA)and non-NMDAsubtypes.
出处
《华西医科大学学报》
CSCD
1994年第1期9-14,共6页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
关键词
脑片
神经元
自发放电
谷氨酸
Rat brain slice Nucleus paragigantocellularis lateralis Neuron spontaneous discharge
L-glutamic acid DL-2-amino-5-phposphonovaleric acidThe Project is Supported by National
Natural Science Foundation of ChinaPresent address:Special Diagnostic Depart
