摘要
本研究用多管微电极技术在大鼠脑片旁巨细胞外侧核（ＰＧＣＬ）区观察了微电泳γ－氨基丁酸（ＧＡＢＡ）及其拮抗剂荷包牡丹碱（ＢＩＣ）对神经元自发放电的效应及ＢＩＣ对ＧＡＢＡ效应的影响。ＧＡＢＡ和ＢＩＣ对自发放电均有兴奋、抑制和无影响三种效应，各占所测试神经无数的５６．１０％，３１．７０％，１２．２０％和６６．６７％，５．８８％，２７．４５％。ＧＡＢＡ的抑制效应和ＢＩＣ的兴奋效应显示量效依赖关系。ＢＩＣ可阻断大多数被试神经元（７８．２６％）对ＧＡＢＡ的反应，本结果从细胞水平上提示ＰＧＣＬ区有内源性ＧＡＢＡ，其神经元上有ＧＡＢＡ＿Ａ受体。
The effects of iontophoretic applicationof γ-aminobutyric acid(GABA) and its antagonist,bicuculline ( BIC)on the spontaneous discharge ofparagigantocellularis lateralis(PGCL ) neurons and theinfluence of BIC on the effects of GABA were studiedin brainstem slice in rat with multibarrel microelec-trode techniques. Both GABA and BIC showed threetypes of effects,i.e.,excitatory, inhibitory and unaf-fected; the percentage of each is 56.10%,31.70%, 12.20%(for GABA) and 66.67%,5.88%, 27.45%(for BIC)of the tested neurons,respectively. The in-hibitory effect of GABA and excitatory effect of BICare dose-dependent.In most of the neurons tested theresponse to GABA could be blocked by BIC. The re-sults at cellular level suggest that there is endogenousGABA in PGCL and that there existsGABA_A recep-tors on some of the PGCL neurons.
出处
《华西医科大学学报》
CSCD
1994年第2期155-158,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
关键词
脑片
神经元
自发放电
氨基丁酸
Rat brain slice Nucleus paragigantocellularis lateralis Neuron spontaneous discharge γ-aminobutyric acid BicucullineThe Project Supported by National Natural Sciences Foundation of China Postgraduate
Special Diagnostic Department
Air Force General Hospi
