摘要
应用细胞内记录研究猫离体胰腺神经节细胞的电生理特性。结果：细胞的静息电位为－５８；５±８．７ｍＶ（下同）；膜的输入阻抗（Ｒ＿ｍ）、时间常数（τ）和膜电容（Ｃ＿ｍ）分别为６８．６±５．１ＭΩ、３．４±０．２ｍｓ和５０．８±３．９ｐＦ；当细胞内注入去极化电流引起的电紧张电位达阈电位水平时，所有的细胞都能爆发锋电位，阈电位在静息电位基础上去极１９．２±０．５ｍＶ，锋电位的幅度、超射值、时程分别为８１．０±１．７ｍＶ、２２．６±０．９ｍＶ和２．９±０．１ｍｓ。继锋电位后还出现一幅度为２０．５±０．８ｍＶ的锋电位后超极化，刺激突触前神经时，在大部分细胞均能记录到快兴奋性突触后电位（ｆ－ＥＰＳＰ）或顺向动作电位。ｆ－ＥＰ－ＳＰ的幅值、时程及神经传导速度分别为８．９±０．７ｍＶ、２５．８±１．９ｍｓ和０．４８±０．０４ｍ／ｓ。此外，在所有细胞都能观察到自发ｆ－ＥＰＳＰ。ｆ－ＥＰＳＰ由乙酰胆硷通过菸硷型受体引起。
The work was carried out to investi-gate electrophysiological properties of neurons of catintrapancreatic ganglia in vitro by means of intracellu- lar recordings .The mean value of resting membranepotential was-58.5±8.7mV with arange from- 45 to 72 mV, The mean values of mem- brane input resistance(R_m),time constant(τ)andcapacity(C_m) were 68.6±5.1MΩ,3.4±0.2ms and50.8±3.9pF (n= 28),respectively. When depolariz-ing electrotonic potentials induced by intracellular in-jection of depolarizing current pulses(0.05-0.5nA,5ms)reached the threshold potential level, all of theneurons could fire action potentials. The threshold.amplitude,overshoot and duration of spikes were 19.2±0.5mV, 81.0±1.7mV,22.6±0.9mV and 2.9±0.1ms(n=35).respectively.The spike was followedby a prolonged afterspike hyperpolarization with am-plitude of 20.5±0.8mV(n= 35). In most of neu-rons,fast excitatory postsynaptic potentials(f-EPSP)or orthodromic action potentials were recorded duringstimulation of nerve trunks attached to the intrapan-creatic ganglia. The mean values of amplitude,dura-tion of the f-EPSP and conduction velocity of thenerves were 8.9±0.7mV, 25.8±1.9ms and 0.48±0.04m/s(n=24),respectively. The ongoing synapticactivity was observed in all of cells. Moreover,f-EPSP.was induced by acetylcholine mediated through nico-tinic receptors.
出处
《华西医科大学学报》
CSCD
1994年第2期159-165,共7页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
关键词
胰腺
神经节
电生理
突触传递
Pancreastic Ganglia Intracellular recording Electrophysiological property Synaptic transmission The Project Supported by National Natural Science Foundation of China The postgraduate of 1988 from WCUMS
