摘要
以湘早籼８号为受体材料，以易于大量获得的鲤鱼精液ＤＮＡ为分子供体，进行浸胚法ＤＮＡ导入水稻实验，研究了ＤＮＡ分子在浸胚过程中的降解情况。通过定时取样后琼脂糖凝胶电泳、紫外吸收光谱扫描分析，发现水稻胚在浸泡过程中能主动降解外源ＤＮＡ分子，４８ｈ后大部分ＤＮＡ分子被降解成小分子片段、单磷酸脱氧核苷酸、碱基甚或无机成分；紫外吸收光谱扫描发现２２０～２４０ｎｍ有明显的增色效应。鉴于浸胚法诱变优势的普遍性及基因导入的固有困难，推测外源ＤＮＡ浸胚过程中降解后的成分对诱变起更大的作用。
The DNA degradation was studied in the experiment of transfering exogenousDNA into the rice embryo by embryo-soaking method with rice embryo as the receptor andfish sperm DNA as the donor.The analysis of the timing samples by agarose electrophoresisand ultraviolet absorption spectrum scanning showed the rice embryo was able to degrade theexogenous DNA.The large DNA melecule was degraded into small ones,dNMPs,bases and/or inorganic elements after 48h’s soaking and the ultraviolet absorption increased apparentlyin 220～224 nm. Considering the advantages of inducing variation by embryo-soaking methodand the difficulty of gene transfer,it was supposed that the digested ingradients play an im-portant role in induced variation.
关键词
水稻
外源
脱氧核糖核酸
降解
paddy
exogenous
deoxyribonucleic acid
degradation
