摘要
本实验采用UDS的检测,判断不同剂量电离辐射对脾淋巴细胞损伤后DNA修复能力的影响。结果显示,大剂量(0.5～6.0Gy)X射线全身照射后24h脾淋巴细胞UDS呈剂量依赖性抑制,剂量效应关系呈线性,无肩区。小剂量(25～250 mGy)X射线单次全身照射后24 h脾淋巴细胞UDS的变化与大剂量效应不同,50 mGy组呈现明显的刺激效应(P<0.05)。低水平Y射线持续照射(剂量率5.4m Gy/6 h·d)4周末,累积剂量130mGy脾淋巴细胞UDS也呈现明显的刺激效应(P<0.02)。实验资料说明,大剂量电离辐射对脾淋巴细胞DNA切除修复能力有抑制作用,而小剂量能产生刺激作用,并且引起刺激的总剂量因剂量率的不同而异。
The effect of different doses of ionizing radiation on the capability of spleen cells in repairing DNA damage by assaying UDS in these cells was reported. It was shown that 24 h after whole body X-irradiation with doses in a range of 0.5 to 6 Gy caused a depression of UDS in a dose-dependent manner, the dose-effect relationship being linear without a shoulder. Single whole body X-irradiation with smaller doses (25-250 mGy) resulted in an apparently different picture showing significant stimulation at 50 mGy (P< 0.05) . Continuous low-level γ-irradiation at a dose rate of 5.4mGy/6 h.d also caused stimulation of UDS in the splenocytes (P<0.02) at the end of 4 week-irradiation, with a cumulative dosage of 130 mGy. The experimental data demonstrated that larger doses may cause depression, while smaller doses may cause stimulation of the capability of excision repair of DNA in the splenocytes, and that the total dosage causing stimulation may be different with different dose rates.
出处
《白求恩医科大学学报》
CSCD
1989年第3期228-231,共4页
Journal of Norman Bethune University of Medical Science
关键词
电离辐射
UDS
DNA损伤修复
Excision repair of DNA UDS Ionizing radiation, low dose Stimulation effect of radiation Dose-effect relationship
