摘要
切取沙打旺无菌苗下胚轴接入固体M5培养基(MS+0.2 mg/1生物素+1 mg/1泛酸钙+1 mg/1 2,4-D+0.7 mg/1 BA+0.2 mg/l NAA)上形成愈伤组织,选用褐绿相间的愈伤组织放入液体CM2培养基(M5+1.3 g/l“1640”)中振荡暗培养。每隔15天换一次液,经过4次换液,用400目网过滤得到大量单细胞。取出单细胞进行双层静止暗培养,每隔7—10天加一次液,一个半月形成小块愈伤组织。然后转入固体M5培养基中增殖,20天形成大块愈伤组织,再转入分化培养基MF2(M5去掉2,4-D)中,一个月左右分化出苗,将苗转入生根培养基,长成完整植株。
Study on plantlet regeneration of Astra-galus adsurgens Pall cv.in monocell cultureis reported in this paper.The hypocotyls ofaseptic seedlings were put onto solid M 5 me-dium (MS+0.2 mg/L Biotin+1 mg/L Cal-cium D-pantothenate+1 mg/L 2,4-D+0.7mg/L BA+0.2 mg/L NAA) to form calli.The brown and green calli were put intofluid CM2 medium (M5+1.3 g/L'1640')for vibration culture,25℃,120 rpm,dark.The monocells of suspention culture were ob-tained after 4 times culture and one time fil-tration (400 apertures),then the monocellswere put into double-deck medium (fluid andsolid CM2) for vibration culture.Calli ofsmall size formed through division of mono-cells after 1.5 months.The calli of smallsize were transferred to solid M 5 medium,calli of big size were formed after 20 days,then the calli were transferred to differentia-tion MF 2 medium (M 5 of no 2.4-D).Budswere differentiated after 20 days,and weretransferred to MS medium (1/2MS+2 mg/LIBA).Lastly,intact plants were eventuallyproduced.And causes of cell clones produced,different morphocytological calli and theirdifferentiation were discussed in this paper.
出处
《实验生物学报》
CSCD
1989年第4期377-383,共7页
Acta Biologiae Experimentalis Sinica
关键词
沙打旺
单细胞培养
再生植株
Astragalus adsurgens.Monocell.Plantlet regeneration.
