摘要
以线粒体为模式体系，研究了低水平化学发光的过程和机制。实验发现在一定浓度范围（蛋白０－１００毫克／毫升）内，线粒体悬液的发光是随浓度的升高而增强。最适宜的测量条件为３５℃和ｐＨ＝７．Ａ。注入外源性ＯＨ和ＯＨ的清除剂证实，ＯＨ可起动脂质过氧化反应，且￣１Ｏ＿２是构成发光的体内光子源。超氧化物歧化酶（ＳＯＤ）对线粒体发光有调节作用。
itochondria was used a sa model system to study the course and mechanism of low-level chemiluminescence. It is discovered that the LCL of mitochondria suspension riseswith its concentration increasing under the definited range (0-100 mg/mL protein). Thebest satisfactory condition is 35℃ and PH=7.4. By adding of exogenous OH and OHcleaner, it is convicted that OH can initiate lipld peroxidation reactions and ￣1O_2 is one ofthe main photon sources. SOD(in vivo) may adjust the LCL from mitochondria.
出处
《感光科学与光化学》
CSCD
1994年第4期312-317,共6页
Photographic Science and Photochemistry
基金
国家自然科学基金
关键词
线粒体
化学发光
羟自由基
超氧阴离子
mitochondria
low-level chemiluminescence
activated oxygen
