摘要
对乳鼠与10月龄成年大鼠的晶状体代谢进行比较。结果表明,年成大鼠晶状体脂类过氧化作用较乳鼠显著高(P<0.05),而非蛋白质就基含量则较乳鼠低约22％(P<0.01),谷胱甘肽过氧化物酶(GSH-Px)活性随鼠龄增长而逐渐下降,成年大鼠GSH-Px活性较18日龄乳鼠降低62％(P<0.01),成年大鼠的谷胱甘肽还原酶活性较18日龄乳鼠低47％(P<0.01),而谷胱甘肽硫转移酶则高14％(P<0.01)。据此结果推测,去年性白内障的发生可能与晶状体抗氧化遗伤的防御机制减弱与脂类过氧化作用增加有关。而乳鼠晶状体谷胱甘肽硫转移酶(GST)活性低于成年大鼠或许是某些中毒性白内障只可在幼年动物诱发成功的影响因素之一。
The amount of LPO and NP-SH, the activities of GSH-Px, GST and GR have, been measured in the lenses of rats 18 days, 22 days and 10 months of age.Rats growing from suckling stage into adult hood, LPO was increased obviously with the age, but NP-SH level was decreased, and the activity of GR in lenses of adult rats showed a decrease of 47% . GSH-Px activity in adult rats was only 38% of that in suckling rats.It may be suggested that the inactivation of these enzymes may be resulted in elevation of LPO and decrease of glutathione level in lenses,and that this may be responsible for the oxidative modification of lens proteins observed in senile cataracts.
关键词
白内障
晶状体代谢
脂类过氧化
Cataract Metabolism in rat lens Senile cataract Lipid peroxida-tion Glutathione
