摘要
本文采用ＭＳ，Ｂ５和ＰＣ一Ｌ２作为基本培养基，在不同激素组合条件下对红三叶（Ｔｒｉｆｏｌｉｕｍｐｒａｔｅｎｓｅ）子叶及下胚轴进行组织培养。在ＭＳ，Ｂ５和ＰＣ一Ｌ２附加２ｍｇ／Ｌ２，４－Ｄ和０．５ｍｇ／ＬＢＡ的培养基上，子叶及下胚轴愈伤组织诱导率均在８９％以上。愈伤组织继代在Ｂ５无机盐十三倍ＭＳ有机成分＋３％蔗糖＋０．５ｍｇ／Ｌ２，４－Ｄ＋０．５ｍｇ／Ｌ６ＢＡ０．２ｍｇ／ＬＮＡＡ，＋１０ｍｇ／ＬＶｃ的培养基上生长良好。愈伤组织在Ｂ５附加０．１ｍｇ／Ｌ６ＢＡ＋０．２ｍｇ／ＬＩＢＡ的培养基上再生植株。下胚轴在ＭＳ附加２ｍｇ／Ｌ６ＢＡ＋１ｍｇ／ＬＮＡＡ的培养基上直接分化形成丛生苗，转入无激素ＮＳ培养基上再生根。实验表明ＶＥ，Ｖｃ，ＰＶＰ和活性碳对愈伤组织褐化有抑制作用，１０ｍｇ／ＬＶｃ抗褐化作用最好。
Cotyledon and hypocotyl of Red Clover( Trifolium pratense L.) were placed respectively on MS,B5,PC- L2 medium containing various combinations of auxins and cytokinins to induce callus forma-tion。 Frequency of callus inducing was over 89%mthe media containing 2mg/L 2,4-D and 0.5 mg/L6BA。 The plant callus when transfering for generations in 3-week time duration to medium Bs containing thr-eefold organic components of MS+ 3%sucrose+0.5 mg/L2,4D+ 0.5mg/L 6BA+0. 2 mg/L NAA+10 mg/L Vc.was growing well.Callus tissue could obtain regenerated plan ts and Shoots after subculturewas placed onto medium containing 0. 1 mg/L 6BA+0. 2 mg/L ABA in B5 basal medium, Moreover,clus-tered shoots were obtained directly from hypocotyl segments on MS medium con tainin g 2 mg/ L 6日A一1mg/LNAA ,and then regenerated shoots were induced on a hormone free MS medium。 In the experiment,itwas indicated that VE.,Vc,PVP and activated charcoal would have some in hibition of browning reaction tocallus,among them,10 mg/LVc was shown to have the most effecti ve action to browning reaction in thisrespect。
出处
《草业学报》
CSCD
1994年第2期51-54,共4页
Acta Prataculturae Sinica
关键词
红三叶
组织培养
再生植株
Red Clover,Tissue culture,Plant regeneration,Inhibition to browning reaction
