摘要
１３例胸腺组织取自３．５至４个月新鲜死亡人胎，剪碎后种在培养瓶内。所用培养液为ＤＭＥ，另加５％胎牛血清、１０％新生牛血清及Ｄ－缬氨酸６５０ｍｇ／Ｌ早期培养中出现两类不同形态的细胞。上皮样细胞的ＮＥ、ＡｃＰ活性及ＰＡＳ反应较弱，ＡＴＰ酶和５－ＮＴ活性较强，角蛋白呈阳性。成纤维样细胞显示较强的ＮＥ、ＡｃＰ活性及ＰＡＳ反应，Ｖｌｍｅｎｔｉｎ呈阳性。３例培养中均含两类细胞，表明培养细胞仍为胸腺基质细胞的混合体。
hymic
tissues(three cases )were obtained from 3.5- to 4- month-old fresh
dead human fetuses. Thetissres were minced into pieces and were
seeded in glass flasks.Cell culture medium is Dulbecco s mo- dified
Eagle s medium(DME) suppiemented with 5% fetal bovine serum and
l0%newborn calf serum and D-valine,650 mg/liter.The polygonal
epithelium-like cell and spindle fibroblast-like cells were present
in early cultures . In epithelium-like cells the activities of
non-specific esterase and acid phospha- tase and PAS reaction
were weak,the activities of ATPase and 5’-nueleotidase were strong,
and kera- tin( AC1)was expressed. Fibroblast- like cells displayed
the high activity of non-specific esterase and acid phosphatase,and
the strong PAS reaction,and the presence of vimentin(AV3). As two
cell types were present in all of the cultures of three cases,it
indicated the cultured cells were a mixture of thymic stromal cells.
出处
《北京医科大学学报》
CSCD
1994年第2期93-95,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金和美国洛氏基金
关键词
细胞培养
胸腺基质细胞
人胎
Cell culture
Thymic stromal cell:Histochemistry
Human fetus
