摘要
以家兔骨骼肌组织提取细胞膜ＤＨＰ受体的粗制品及提纯制品，经聚丙烯酰胺凝胶电泳。结果表明，骨骼肌细胞膜ＤＨＰ受体蛋白亚单位的分子量为１７０、１４０、５５和３３ｋ。采用３ＨＰＮ２００－ｌｌ０与ＤＨＰ受体进行受体结合实验，其ＫＤ为２，５ｎｍｏｌ／ＬＢｍａｘ为５８．９ｐｍｏｌ／ｍｇ。人参单体皂甙Ｒｂ１表现出抑制ＤＨＰ受体的作用，ＩＣ５０为１２０ｕｇ／ｍｉ而人参单体皂甙Ｒｇ１则无明显作用。
The Dihydropyridine(DHP)receptors were purified from rabbit skeletal muscle transverse tubulemembranes using Chromatography on WGA-Sepharose,DEAE-Sephadex Chromatography and Sucrosegradient sedimentation. The Bmax and KD for 3H PN 200-110 binding to the T-tubule membranes are58.9 pmol/mg and 2.5 nmol/L respectively.The purified receptor proteins in SDS polyacrylamide gelelectrophoresis showed at least four polypeptides.These polypeptides have molecular weight of l70,140,55 ,and 33K. Ginsenoside Rb1 showed calcium antagonistic activity in the 3H PN200- 110 To theDHP recetpor binding assay.The IC50 of Ginssenoside Rb1 was l20 mg/ml but Ginsenoside Rg1 did notshow Calcium antagonistic activity in the same experiment
出处
《北京医科大学学报》
CSCD
1994年第2期90-92,共3页
Journal of Peking University(Health Sciences)
基金
自然科学基金
关键词
人参
皂甙
DHP受体
钙通道阻滞剂
Dihydropyridine receptor
Ginsenoside Rb1
Ginsenoside Rg1
