摘要
本文用火箭免疫电泳法测定了72例正常人血清载脂蛋白A-Ⅰ、A-Ⅱ(Apo A-Ⅰ、A-Ⅱ)的水平,并与单向免疫扩散法作了比较,结果显示火箭免疫电泳法测定的Apo A-Ⅰ水平略高于单向免疫扩散法,而Apo A-Ⅱ的水平相似。相关回归分析显示两种方法测定Apo A-Ⅰ和Apo A-Ⅱ的r值分别为0.6568和0.4779(P均<0.001),有极密切相关。同时Apo A-Ⅰ水平均与HDL-C水平呈正相关。在高胆固醇血症组,电泳法测得的Apo A-Ⅰ水平和对照组相似,也与其血清HDL-Ch水平无改变相符,而免疫扩散法则低0.06g/L。高甘油三酯血症组的HDL-Ch水平显著低于正常,电泳法所测得的Apo A-Ⅰ示显著低于正常,但免疫扩散法的差值无显著意义。两种方法的准确性、精密性及灵敏度的比较提示前种方法测定Apo A-Ⅰ、Apo A-Ⅱ优于后种。
Rocket electroimmunoassay (REA) and the established technique of radial immunodiffusion (RID) were used for estimation of serum apolipoprotein A-Ⅰ and apo A-Ⅱ, the major protein components of high density lipoprotein (HDL). The withinbatch and between-batch coefficients of variation in apo A-Ⅰ for REA were 2.9% and 4.8%, and for RID 3.8% and 6.5% respectively. Reproduction and sensitivity of REA were found better than those of RID. Sera from 72 healthy subjects (36 each of male and female) and 42 patients with hyperlipoidemia were analysed by both methods and the results showed that for apo A-Ⅰ r=0.6568, A-Ⅱ r=0.4779 (P<0.001). Both assays were found closely correlated with HDL-Ch, but the variance of apo A-Ⅰ in hyperlipoidemia could not be clearly distinguished by RID. Rocket electroimmunoassay is therefore considered to be satisfactory for estimation of serum apo A-Ⅰ and apo A-Ⅱ.
出处
《上海医学》
CAS
CSCD
北大核心
1989年第6期338-342,共5页
Shanghai Medical Journal
