摘要
逆转录聚合酶链式反应(RT-PCR)分别对119例心肌炎患者,37例正常人血标本进行肠道病毒核糖核酸(RNA)检测,检出率分别为49.6％及5.4％。患者血清柯萨奇B组病毒中和试验阳性率为21.8％。表明RT-PCR是检测肠道病毒感染的一种敏感性高、特异性强、简便快速的方法,优于中和试验。
Plasma or peripheral leucocyte specimens from 119 patients suspected of myocarditis and 87 normal individuals were assayed for enteroviruse RNA by reversetranscription polymerase chain reaction (RT-POR). Lecocyte and plasma epecimens were tested simultaneously in 40 of these patients. Standard strains of Coxsackieviruse B (CVB) were used as positive controls. Specific amplified products were acquired from all six OVB strains. Positive rates for perpheral blood leucocyte specimens from 119 patients and 87 normal individuals were 49.6 % and 6.4% respectively. The positive rate of the neutralization test in patients was only 21.8%. Positive rates for enterovirus RNA in plasma and leucocyte specimens from 40 patients were 20.0% and 56.0% respectively. It is suggested that RT-POR should be a specific, sensitive, convenient and rapid method for the detection of enterovirus infection.
出处
《上海医科大学学报》
CSCD
1993年第6期472-474,T025,共3页
Journal of Fudan University(Medical Science)
关键词
聚合酶链反应
肠道病毒
RNA
polymerase chain reaction
enterovirus
ribonucleic aoid
myocarditis
