摘要
大鼠经松节油注射,48h后血浆中产生高浓度的应激相α_2-巨球蛋白(Apα_2M),它与天然的α_2M无差异。纯化分三步:去低密度脂蛋白、Sephaeryl S-300凝胶过滤及反相亲和层析。最后得到高纯度的Apα_2M。制备其抗体,并用火箭免疫电泳法测定大鼠血浆α_2M含量。
A modified simple method of production of rat acute-phase plasma as-macroglo-bulin (Apα2M) by turpentine is reported. Purification of Apα2M involved three steps: (1) elimination of low density lipoproteins by dextran sulphate; (2) separation by gel filtration on Sephaoryl S-300; and (3) highly purified Apα2M obtained by negative affinity chromatography.Pure Apα2M was measured by rocket immunoeleotrophoresis assay and usee to prepare antiserum.
出处
《上海医科大学学报》
CSCD
1989年第1期59-62,共4页
Journal of Fudan University(Medical Science)
