摘要
本文对苏云金杆菌（Ｂｔ）制剂的生物测定标准化及目前采用的生物测定方法作一较详细的介绍。其中对鳞翅目幼虫的生物测定包括混合饲食法、浸叶饲喂法、蜡洞法、液滴法；对双翅目幼虫的生物测定则有世界卫生组织提出的方法和美国国内标准方法；对路翅目幼虫目前还没有一个统一的标准方法。生化和免疫测定法比传统的生物测定方法快速、简便，但亦存在不足。
This review dealt with the importance and Popular procedures of the standardized bioassay for Bacillus thuringiensis. The standardized bioassay procedures for larvae of Lepidoptera includes: the diet - incorporation bioassay, the surface contamination assay, the hole - in - wax assay and the droplet assay. The standardized bioassay procedures for larvae of Diptera are the World Health Organization method and the U. S. method. However the bioassay for larvae of Coleoptera has not ho standardized. Though immunochemical assays have some defects, they are more rapid and simple than conventional bioassays.
出处
《昆虫天敌》
CSCD
2000年第2期83-88,共6页
Natural Enemies of Insects
基金
国家自然科学基金资助项目(39900098)
广东省自然科学基金资助项目(980296)
