摘要
为了观察猴艾滋病D型逆转录病毒Ⅰ型(SRV1)感染和未感染的Raji细胞同工酶活性的变化,用超声波破碎细胞,提取细胞内总蛋白,然后用垂直薄层聚丙烯酰胺凝胶等电聚焦电泳对其进行酶谱分析。 以α-醋酸萘酯和/或β-醋酸萘酯为底物时,SRV1感染的Raji细胞近酸性端酯酶Est 11-14的活性是未感染Raji细胞的2.2倍,提示酯酶活性的变化与病毒感染有关。SRV1感染和未感染Raji细胞系的酸性磷酸酶的酶带数,pI值和酶活性无差异,二者均没有检出乳酸脱氢酶(LDH)、醇脱氢酶(ADH)、过氧化物酶(POD)、苹果酸脱氢酶(MDH),但有较弱的碱性磷酸酶(APH)。这些结果提示酯酶活性的增加有可能作为SRV1感染Raji细胞的一种指标。
In order to compare the activities of isozymes in Raji cells infected by simian AIDS type D retrovirus serotype 1 (SRVI ) with that in noninfected cells, the total cell proteins were extracted by ultrasonic vibrator and the enzyme pattern was analyzed with isoelectric focusing in a vertical polyacrylamide gel.When alpha -naphthyl acetate and / or beta -naphthyl acetate were used as substrates,in acid side the activity of Est 11-14 of Raji cells infected by SRVI was 2. 2 times as much as noninfected Raji cells, indicating that the increase of esterase activity was associated with virus infection. There were no differertces in enzyme band number,their pi and activity of acid phosphatase between SRV 1-infected-and noninfected Raji cells. Activities of LDH.ADH.POD and MDH in these cells were not detectable, but APH was at low activity. These results suggest that the increase of esterase activity may be considered as a marker for SRV 1-infected Raji cells.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1993年第5期2-4,共3页
Chinese Journal of Zoonoses
基金
中国科学院重大应用项目基金
云南省应用基础研究基金
关键词
艾滋病
艾滋病毒
RAJI细胞
同功酶
Simian AIDS,SRV1 , Isozymes.Raji cell,Isoelectric focusing
