摘要
本文以结晶紫染料摄入法,检测52例寻常型银屑病患者内毒素诱导的外周血单个核细胞(PBMCs)产生肿瘤坏死因子(TNF)活性,并用T 细胞表型(T_1、T_4、T_8)单克隆抗体间接免疫荧光法,检测患者外周血T 细胞及其亚群的百分率。结果寻常型银屑病患者外周血TNF 诱生活性(6.13±2.41U/ml)、T_4细胞百分率(46.31±6.31%)及T_4/T_(?)比值(1.54±0.27)均显著低于正常人对照组(P<0.01),而T_(?)细胞百分率(30.33±2.12%)则明显增高(P<0.01)。表明银屑病患者存在细胞免疫功能缺陷;TNF 产生能力低下和T_4/T(?)的比例失调与银屑病的发病密切相关。
The tumor necrosis factor (TNF) activity induced by peripheral blood mononuclear cells
(PBMCs) and T cell subsets including T_1,T_4 and T_8 form 52 cases with psoriasis have been sti-
multaneously detected with the L929 cell cytotoxic assay and monoclonal antibody indirect im-
munofluenscence method,respectively.The results indicated that the TNF activity (6.13±2.
41u/ml),the percentage of T_4 cell (4.63±6.31%) and ratio of T_4/T_8 cell (1.54±0.27) in
psoriasis were all lower than that of in normal individual (P<0.01),but the percentage of T_8
cell (30.33±2.12%) in psoriasis increased as compared with normal cortro control (P<0.01).
The results suggest that thsrs is deficiency of cellular immunity function in psoriasis and the de-
crease of TNF inducing ability and the derangement of T_4/T_8 subsets are closely related to the
pathogenesis of psoriasis.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1993年第4期246-248,共3页
Chinese Journal of Immunology
关键词
银屑病
肿瘤坏死因子
T细胞
Psoriasis Tumor Necrosis Factor T Cell Subsets
