摘要
目的 :探讨痢疾杆菌L型的诱导方法 ,比较原菌及L型的生物学性状。方法 :用抗生素纸片法、胆盐、胆汁法分别诱导痢疾杆菌D15为L型 ,对原菌及L型菌的形态、染色、培养特性、生化反应及对药物的敏感性进行比较。结果 :用抗生素纸片法、胆盐、胆汁法均可诱导痢疾杆菌D15为L型 ,稳定的L型在形态、染色、培养特性、生化反应等方面均与原菌有明显区别 ,回复株则与原菌基本相同。结论 :痢疾杆菌在诸多因素作用下可发生L型变异 ,L型菌与原菌性状不同 。
Objective:To explore the inducing method of Shigella dysenteriae (S.D) L forms and compare different biological features of S.D and its L forms.Methods:S.D D 15 was treated with antibiotic,sodium desoxycholate and bile to induce its L form.S.D original form and its L forms were compared in their morphology,stain,culture feature,biochemical reaction,and its antibiotic susceptibility.Results:By using these three methods,S.D D 15 were induced into D 15 L form.The stable L form of S.D was very different from its origical form in morphology,stain,culture feature,biochemical reaction,and antibiotic susceptibility.Meanwhile,compared with origical form,the reversional form was not significantly changed.Conclusions:S.D could be induced into its L form by different factors,L form and its origical forms' feature were very different,the L form of S.D must be paid an attention to by clinicians.
出处
《蚌埠医学院学报》
CAS
2001年第4期283-285,F003,共4页
Journal of Bengbu Medical College
基金
安徽省教育厅自然科学研究资助项目!(95JL0 10 2 )
