摘要
研究了外源2,4-D、ZT,6-BA和ABA对石防风(Peucedanum terebinthaceum Fisch.)体细胞胚发生及发育的影响,以及内源ABA在体细胞胚发生发育过程中含量的变化。内源ABA的含量在单细胞阶段较高,细胞团及胚性细胞团时下降,球形胚时剧烈增高,随着胚的发育又下降。这种变化可以说明外源ABA在各个阶段所起作用不同的原因。
The influence of different plant growth regulators including 2,4-D,ZT, 6-BA and ABA on somatic embryogenesis and the amount of endogenous ABA at different stages of embryogenesis was investigated. The effect of each plant growth regulator changed according to the stage of embryogenesis. The amount of endogenous ABA was rather high in single cell stage, decreased at cell clump and embryogenic cell clump stages and dramatically increased at globular embryo stage, It decreased again as the embryo developed. This change in amount of the endogenous ABA explained very well the difference in the effect of exogenous ABA when applied at different stages of embryogenesis.
关键词
石防风
体细胞胚发生
生长调节物质
Peucedanum terebinthaceum
Somatic embryogenesis
Plant growth regulators
Endogenous ABA
