摘要
目的 检测雌激素硫酸转移酶 (EST)在乳腺组织中的表达 ,探索其在乳腺癌变中的意义及其与雌激素的关系。方法 2 4例乳腺癌、6例正常乳腺和 7例植入裸鼠后给苯甲酸雌二醇的人正常乳腺组织 ,用逆转录多聚酶链式反应 (RT PCR)方法检测雌激素硫酸转移酶 (EST)mRNA的表达 ,并用免疫组织化学的方法检测乳腺癌组织中雌激素受体 (ER)的蛋白表达。结果 正常乳腺组织表达ESTmRNA ,给苯甲酸雌二醇的植入乳腺中其表达升高 ,但乳腺癌组织中ESTmRNA表达降低或缺失。结论 乳腺癌组织ESTmRNA的表达降低或缺失 ;正常乳腺EST受雌激素调节。
Objective To detect the expression of estrogen sulfotransferase (EST) in breast tissues, and to investigate its significance in breast carcinogenesis and the relationship between EST mRNA and estrogen. Methods Seven specimens of normal human breast tissues from patients with benign breast diseases were implanted subculaneously into athymic nude mice, one specimen divided into many pieces to be implanted into 2 mice, fotally 14 mice. Then the 14 mice were divided into 20 groups: as control and the other injected intramuscularly with estradiol benzoate (B-E 2) every other day for 6 weeks. Six weeks after, the implanted tissues were taken out. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of EST mRNA in 24 specimens of breast cancer, 6 specimens of normal breast tissues around the breast fibroadenoma, all resected during operation, and the specimens of implanted normal breast tissues from the mice (control group and B-E 2 group). The protein expression of estrogen receptor (ER) was detected by immunohistochemistry in the 24 specimens of breast cancer. Results EST mRNA was expressed in all normal human breast tissues and the expression was increased in the grafts from mice of B-E 2 group. However, the specimens of breast cancer showed decrease or absence of EST mRNA expression. Conclusion EST mRNA expression is decreased or lost in breast cancer tissues. EST mRNA can be regulated by estrogen in normal breast tissues.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2003年第21期1891-1894,共4页
National Medical Journal of China
