摘要
将表面增强拉曼光谱 (SERS)的高度灵敏性应用于标记免疫检测 ,具有很大的意义[1] 。在“固相抗体 待测抗原 标记抗体”夹心复合物体系中 ,以 (SERS)标记的金溶胶与抗体结合 ,制备标记抗体。以芳香族化合物苯硫酚为标记分子 ,与一定大小粒径的金纳米粒子形成S—Au键 ,生成带有SERS信号的标记金溶胶。表面带负电荷的标记金溶胶与带有正电荷基团的抗体形成牢固的标记免疫金溶胶。从金纳米粒子粒径的选择、在金溶胶中加入苯硫酚的量及反应时间、抗体对标记金溶胶标记分子的SERS信号的影响进行了研究。
The applications of the high sensitivity of Surface-Enhanced Raman Spectroscopy (SERS) in labeled immunoassay have proved to be very useful. In the sandwich structure of 'immobile antibody-antigen-labeled antibody', the authors prepared the labeled antibody by attaching gold nanoparticles, which was first labeled with molecule that had SERS signal, to the antibody. In this article, colloids were labeled with Raman reporter thiophenol through S-Au bond on gold and formed surface-enhanced Raman scattering (SERS) reporter-labeled gold colloids. Then, goat anti-rat antibody was adsorbed onto the reporter-labeled gold colloids and formed reporter-labeled immunogold colloids. This paper studied factors affecting FT-SERS reporter-labeled immunogold colloids such as the sizes of gold particles, the amount of thiophenol in gold colloids and processing time, and whether goat anti-rat antibody would influence the SERS of reporter-labeled gold colloids.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第12期1575-1578,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金 (2 0 2 730 4 5
2 0 3730 4 6)
厦门大学固体表面物理化学国家重点实验室开放课题资助项目
