摘要
目的探讨病毒感染与慢性阻塞性肺病(COPD)的关系及在其发病机制中的作用。方法收集了COPD急性发作期患者86例(A组)和COPD稳定期患者32例(B组),采用生物薄片技术对患者血清进行了呼吸道合胞病毒(RSV)、腺病毒(Adv)、柯萨奇病毒(Cox)、巨细胞病毒(CMV)特异性抗体IgG、IgM检测。同时采用碱性磷酸酶———抗碱性磷酸酶(APAAP)桥联酶标法检测了T淋巴细胞亚群CD3,CD4,CD8。结果两组患者RSV,Adv,Cox和CMV特异抗体IgM,IgG阳性率比较差异无显著性(P>0.05);两组患者T淋巴细胞亚群CD3,CD4,CD8比较差异有显著性(P<0.01 or P< 0.05);在A组,病毒抗体阳性组与病毒抗体阴性组T淋巴细胞亚群CD3,CD4比较差异有显著性(P<0.01 or P<0.05),而CD8间比较差异无显著性(P>0.05)。结论病毒感染是COPD急性加重期的重要诱因,而且病毒感染与COPD发病具有相关性。
Objective: To study the relationship between viral infection and chronic obstructive pulmonary disease (COPD), and the role of viral infection in the pathogenesis of COPD. Methods: Serum samples from 86 patients with acute exacerbation of COPD (Group A), 32 patients with stable COPD (Group B) were tested for the specific IgG and IgM for Respiratory Syncytial virus (RSV), Adenovirus (ADV), Coxsackievirus (COX) and Cytomegalovirus (CMV) with Biochip technique-indirect immunofluorescence test. T lymphocyte subsets (CD3, CD4, CD8) were studied by Alkaline phosphatase anti-alkaline phosphatase (APAAP). Results: The positive rates of IgM and IgG were no significant difference between group A and group B (P >0.05). In group A, the expression of CD3, CD4 and CD8 were significantly lower than those in group B (P <0.01 or P <0.05); In group A, the expression of CD3, CD4 in viral antibody positive groups were significantly lower than those in viral antibody negative groups (P <0.01 or P <0.05), but the expression of CD8 were not significantly different (P >0.05). Conclusions: The viral infection was common in acute exacerbation of COPD, and related to the pathogenic mechanism of COPD.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第24期22-24,28,共4页
China Journal of Modern Medicine
关键词
肺疾病
阻塞性
病毒
T淋巴细胞亚群
pulmonary disease
obstructive
virus
T lymphocyte subsets
