摘要
目的 研究间充质干细胞(mesenchymalstemcell,MSC)对T细胞的免疫调节作用。方法 从骨髓中分离培养MSC ,从外周血中分离单个核细胞 ,进一步用免疫磁珠纯化CD3+ T细胞 ,观察MSC对T淋巴细胞转化的影响 ;且用ELISA实验检测上清中IFN γ和IL 10的表达。结果 当CD3+ T细胞量为 2× 10 5/孔时 ,MSC对其增殖反应主要表现为负调控 ,且随MSC细胞量的增多 ,这种负调控越明显 ;而当体系中CD3+ T细胞量为 5× 10 4 /孔时 ,MSC对其增殖反应表现为正调控 ,而且随MSC细胞量的增多这种促进作用越明显。ELISA结果表明 ,在没有MSC的存在下 ,2× 10 5/孔的T细胞分泌细胞因子IFN γ和IL 10均高于 5× 10 4/孔的T细胞 ;但在有MSC存在情况下 ,2× 10 5/孔的T细胞分泌细胞因子IFN γ和IL 10反而低于 5× 10 4/孔的T细胞。结论 MSC对T细胞的调控是复杂的 ,不仅与MSC的细胞量有关 ,还与T细胞的量有关。
Objective To study the immunoregulatory ability of mesenchymal stem cell (MSC) on T cells. Methods MSC were isolated and cultured from bone marrow and CD3 + T cells from peripheral blood. Then the action of MSC on T cell proliferation was investigated. ELISA assay tested IFN-γ and IL-10 secretion of CD3 + T cells. Results The data showed that MSC inhibited T cell proliferation in a dose dependent manner when there was 2×10 5/well (96-well plate) CD3 +T cells. While MSC stimulated T cell proliferation when there was 5×10 4/well CD3 + T cells. High dose CD3 +T cells produced high level IFN-γ and IL-10 in the absence of MSC, while when there were MSC in the culture, 5×10 4/well CD3 +T cells secreted more IFN-γ and IL-10 than 2×10 5/well CD3 + T cells. Conclusion The result suggested that the effect of MSC on T cells was complex, not only correlating with the cell concentration of MSC but also of T cells.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2005年第2期130-132,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金 (编号 30 2 71 2 4 5
30 4 70 640 )
国家 973资助(编号G1 9990 5430 2 )
国家 863 (编号 2 0 0 3AA2 0 51 70 )资助课题
