摘要
本研究旨在探讨CD8+ CD2 8- T细胞在间充质干细胞 (MSC)抑制T细胞增殖中的作用。应用尼龙毛柱分离T淋巴细胞 ,再经CD8磁珠分选出CD8+ T淋巴细胞 ;用植物血凝素 (PHA)刺激与或未与MSC共培养 3天的CD8+ T细胞 ,应用MTT法测定T细胞的增殖 ;应用流式细胞术分别检测与或未与MSC共培养 8天的CD8+ T细胞亚群的变化及用PHA刺激与或未与MSC共培养 3天的CD8+ T细胞亚群的变化。结果表明 ,MSC抑制PHA刺激引起的CD8+ T细胞的增殖 ,且这种抑制作用是剂量依赖性的 ;流式细胞术结果显示 ,与未经MSC处理的CD8+ T细胞相比 ,MSC显著上调共培养 8天的CD8+ T细胞中的CD8+ CD2 8- 亚群 ,及经PHA作用 72小时后的CD8+ T细胞中的CD8+ CD2 8- 亚群。结论 :MSC通过上调CD8+ CD2 8- T细胞发挥抑制作用。
The aim of this study was to explore effect of CD8+CD28-T-lymphocyte in the inhibition of mesenchymal stem cells (MSC) on T-lymphocyte proliferation. T cells were harvested by using nylon column and CD8+T cells were sorted by magnetic beads; the T-lymphoc yte proliferation in the presence of PHA was evaluated by MTT; the proportion of CD8+CD28-T cells was assayed by fluorescence-activated cell sorter (FACS). The results showed that MSC inhibited T-lymphocyte proliferation and the inhib itory eff ect depended on the amount of MSC; the data of FACS indicated that in the CD8 +T cells co-cultured with MSC, CD8+CD28-T cells were up-regulated signific antly, com pared with the non-treated CD8+T cells. In conclusion, MSC perform their imm unosuppressive function by up-regulation of CD8+CD28-T cells.
出处
《中国实验血液学杂志》
CAS
CSCD
2004年第5期666-669,共4页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目 (编号 3 0 0 70 2 84)
