摘要
使用硫酸铵分级沉淀、Sephadex G-100凝胶过滤色谱及DEAE-Sephadex A-25(A-50)离子交换色谱等分离技术,从黑曲霉(Aspergillus niger)培养液中分离到7种内切β-葡聚糖酶组分,分别称为Ⅰ-2a、Ⅰ-2b、Ⅰ-2c、Ⅰ-2d,Ⅱa、Ⅱb和Ⅲa。经凝胶电泳鉴定均为单一带。
Through systematical isolation and purification of cellulase from Aspergillus niger using the technique of (NH_4)_2SO_4 fraction ation, gel filtration and ion exchange chromatography, we obtained seven components of endo-β-glucanase (Ⅰ-2a,Ⅰ-2b,Ⅰ-2c, Ⅰ-2d, Ⅱa, Ⅱb, Ⅲa) All of them showed homogeneity in the dise gel electrophresis.
出处
《微生物学通报》
CAS
CSCD
北大核心
1993年第1期13-16,共4页
Microbiology China
关键词
黑曲霉
内切葡聚糖酶
纤维素酶
Aspergillus niger
Endo-β-glucanase
Isolation and purification
