摘要
目的:了解维生素D3辅助治疗幼年类风湿性关节炎(JRA)前后外周血单个核细胞(PBMC)体外诱生γ干扰素(IFN-γ)、白细胞介素4(IL-4)水平。方法分别采集维生素D3治疗前后静脉血,分离PBMC,用含20％小牛血、植物血凝素的RPMI-1640培养液,37℃、5％CO2孵箱中培养后,运用BA-ELISA检测INF-γ和IL-4水平。结果:维生素D3治疗4-8w后IFN-γ诱生水平明显降低(P<0.05),而IL-4诱生水平略有增加,IFN-γ／IL-4比值接近正常水平。结论:维生素D3辅助治疗JRA可以使Th1细胞产生IFN-γ受到抑制,Th1／Th2应答失调得到矫正。
Objective To investigate the levels of IFN-γ and IL-4 in peripheral blood mononuclear cells(PBMCs) in vitro before and after receiving vitamin D3-assisted therapy in children with JRA. Methods Venous blood were obtained before and after receiving vitamin D3 therapy respectively. PBMCs separated from the venous blood were cultured in RPMI 1640 containing 20% calf blood and PHA. The levels of IFN-γ and IL-4 in the cultured PBMCs were determined by BA -ELISA. Results The level of IFN-γ was significantly decreased 4-8 weeks after the treatment by vitamin D3(p <0.05), while the level IL-4 slightly increased, and the ratio of IFN-γ to IL-4 came near normal level. Conclusion Vitamin D3-assisted therapy in children with JRA could inhibit Th1 type cytokine from producing IFN-γ and the enables responsive imbalance of Th1/Th2 to be redressed.
出处
《华南国防医学杂志》
CAS
2004年第4期15-17,共3页
Military Medical Journal of South China
