摘要
目的探讨甘糖酯(PGMS)对糖尿病大鼠肾脏细胞间黏附分子-1(ICAM-1)、血管细胞间黏附分子-1 (VCAM-1)表达的影响和保护作用。方法链尿佐菌素(STZ)诱发大鼠糖尿病。甘糖酯(52,26mg·kg-1· d-1)治疗8周,测定血糖、糖化血红蛋白、肾脏/体重,24h尿蛋白排泄率,利用免疫组织化学、逆转录聚合酶链 式反应(RT-PCR)和蛋白印迹(WesternBlot)技术检测肾脏组织ICAM-1,VCAM-1分布及表达。结果与糖 尿病模型组比较,PGMS有减轻肾脏肥大趋势;降低尿蛋白排泄率(P<0.05);免疫组化显示PGMS能明显减 少ICAM-1,VCAM-1在肾脏组织的分布;RT-PCR,Westernblot结果显示PGMS能明显降低ICAM-1, VCAM-1mRNA和蛋白水平表达,高剂量组效果优于低剂量组但无统计学差异。ICAM-1,VCAM-1表达与 肾脏肥大、尿蛋白存在部分正相关。结论PGMS能降低STZ诱发的糖尿病大鼠肾脏ICAM-1,VCAM-1mRNA和蛋白水平。
Objective To observe the effects of propylene glycol mannate sulfate(PGMS) on the expression of ICAM-1 and VCAM-1 in kidney of diabetic rats, and explore the protective effect of PGMS on kidney. Methods Diabetes was induced by injecting STZ. Then the rats were randomly divided into four groups: normal control group, diabetic modal group, treatment group with high-dose and low-dose of PGMS. 8 weeks later, the blood glucose concentrations, HbA1c, the ratio of kidney weight to body weight, urinary albumin excretion rate (UAE ) in 24 hours were examined. The ICAM-1 and VCAM-1 located were detected by immunohistochemistry, while the expression of ICAM-1 and VCAM-1 were detected by Western blot method and the levels of ICAM-1 and VCAM-1 mRNA by RT-PCR.Results PGMS could reduce kidney hypertrophy and lower urinary albumin excretion rate (UAE)of 24-hour significantly. Immunohistochemical study with light microscopy demonstrated that the levels, both ICAM-1 and VCAM-1 in the kidney of test groups decreased obviously. PGMS down-regulated the mRNA and protein levels of ICAM-1 and VCAM-1 significantly . A positive correlation between expression of ICAM-1,VCAM-1 and kidney lesion was observed. Conclusion: PGMS decreased the expression of ICAM-1 and VCAM-1 in diabetic rats.
出处
《中国海洋药物》
CAS
CSCD
2005年第1期10-16,共7页
Chinese Journal of Marine Drugs
基金
山东省科技厅资助项目(1999BB1DBA4)
关键词
甘糖酯
黏附分子
糖尿病肾病
尿微量白蛋白
propylene glycol mannate sulfate
adhesion molecule
diabetic nephropathy
microalbuminuria
