摘要
建立了用反相高效液相色谱法测两歧双歧杆菌 DNA G+ C 摩尔百分含量的方法. 采用 20 mmol·L-1KH2PO4(pH 5 6)缓冲液—甲醇(体积比90∶10)为流动相, 检测波长260 nm, 流速1 mL·min-1, 在Kromasil C18柱(5μm, 250 mm×4.6 mm i d )上对各碱基进行分离, 以峰面积计算, 计算值和文献值相符.
A method was established to determine the G+C mol% of DNA in Bifidobacterium bifidum. Various bases were separated on a Kromasil C_(18) (5 μm, 250 mm×4. 6mm i. d. ) column by using KH_2PO_4 buffer (20 mmol·L^(-1), pH 5. 6)-methanol(90∶10) as mobile phase at a flow rate of 1 mL·min^(-1). The UV detection was set at 260 nm. The calculative value was identical with the documental value after the calculation by making use of peak areas.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第1期96-100,共5页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(30200244).
关键词
两歧双歧杆菌
G+C摩尔百分含量
反相高效液相色谱
bifidobacterium bifidum
G+C mol%
reversed-phase high performance liquid chromatography
