摘要
目的:测定妊娠高血压综合征(妊高征)患者血液血管内皮生长因子 (VEGF)、肿瘤坏死因子 (TNF)、胎盘一氧化氮(NO)的含量。方法:选择妊高征患者 46例,其中轻度 14例,中度 15例,重度 17例;选择同期正常晚期妊娠妇女 16例为对照。采用酶联免疫吸附法测定各组孕妇血清VEGF水平,放射免疫法测定血浆TNF α含量,用硝酸还原法测定胎盘组织NO浓度。结果:①轻度妊高征患者血清VEGF水平及胎盘组织NO含量与对照组相比,差异无统计学意义(P>0. 05),中、重度妊高征患者 2指标均低于对照组 (P均 <0. 05)。②各组妊高征患者血浆TNF -α水平均较对照组明显增高(P<0. 05),其中以中重度增高明显。③妊高征孕妇血清VEGF水平与胎盘组织NO含量呈正相关(r=0. 68, P<0. 01),而TNF- α水平与NO含量呈负相关 (r=-0. 71, P<0. 01 )。结论:血清VEGF、TNF的改变在妊高征发病中起重要作用,它们还可通过影响NO的合成进一步在妊高征发病中发挥作用。
Aim: To detect the serum vascular endothelial growth factor(VEGF), tumor necrosis factor-α(TNF-α), and nitric oxide(NO) in placeatal tissue of patients with pregnancy induced hypertension(PIH). Methods: A total of 46 women with PIH were enrolled for this study,while 16 normal term-pregnant women were chosen as control group.Serum VEGF level was measured by ELISA.Serum TNF-α concentration was measured by radioimmunoassay.The concentration of NO in placental tissue was assayed using nitrite reductase. Results: There was no difference between the mild PIH and normal group in the VEGF level and NO concentration, but the VEGF level and NO concentration in moderate and severe PIH were significantly lower than those of normal group (P<0.05). The concentration of TNF-α in PIH group was significantly higher than that of control group (P<0.05). The serum VEGF level was positively correlated with the concentration of NO in placental tissue(r=0.68,P<0.01), and there was a negative correlation between the serum TNF-α concentration and the NO concentration in placental tissue(r=-0.71,P<0.01). Conclusion: The changes of VEGF and TNF might play an important role in the pathogenesis of PIH,which can further affect the synthesis of NO.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2005年第1期83-85,共3页
Journal of Zhengzhou University(Medical Sciences)
关键词
妊娠高血压综合征
血管内皮生长因子
肿瘤坏死因子
一氧化氮
pregnancy induced hypertension
vascular endothelial growth factor
tumor necrosis factor
Nitric Oxide
